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T8665
Sigma
3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System
Description
| Application | TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. |
| Caution | This substrate is light-sensitive and should be protected from direct sunlight or UV sources. |
| Physical form | Ready-to-use. |
Properties
| EQP level | Premium |
| storage temp. | 2-8°C |
Safety
| Hazard Codes | T |
| Risk Statements | 10-23/24/25-39/23/24/25 |
| Safety Statements | 7-16-36/37-45 |
| RIDADR | UN 1993 3/PG 3 |
| Flash Point(F) | 130 °F |
| Flash Point(C) | 54 °C |
